Turnover of messenger RNA in transplantable hepatomas and host liver of rats.

Turnover of Messenger RNA in Transplantable Hepatomas and Host Livers of Rats1

The turnover of messenger RNA (mRNA) in two intrahepatically transplantable hepatomas (5123 and 19) and host livers of Buffalo rats was evaluated with four different approaches. [14C]Oroticacid incorporation into the rapidly labeled peak between 18S and 4S of total polyribosomal RNA was measured, in vitro RNase assay of [14C]orotic acid-labeled mRNA of polyribosomes was utilized. The decay of m...

Effect of Nutritional Alterations on Protein Synthesis in Transplantable Hepatomas and Host Livers of Rats1

studies, we investigated the effects of: (a) a single feeding of a complete mixture of amino acids on individual amino acids to fasted animals (21 , 24, 27); (b) a single feeding of a purified diet devoid of protein on amino acids (23); (c) force feeding a purified diet devoid of single essential amino acids for 1 to 3 days (16). Under these experimental conditions, hepatic pro tein synthesis w...

Effect of nutritional alterations on protein synthesis in transplantable hepatomas and host livers of rats.

Inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in Morris hepatoma 7800 after intravenous injection of mevalonic acid.

The effect of i.v. injection of mevalonate on the activity of microsomal 3-hydroxy-3-methylglutaryl Coenzyme A reductase was studied in livers from non-tumor-bearing rats and in host liver and hepatomas from rats bearing transplantable Morris hepatoma 7800. We confirmed that a single bolus injection of 100 mg of mevalonate in non-tumor-bearing male rats caused a 90% inhibition of hepatic 3-hydr...

Activities of some enzymes of pyrimidine and DNA synthesis in a rat transplantable hepatoma and human primary hepatomas, in cell lines derived from these tissues, and in human fetal liver.

Activites of the enzymes DNA polymerase, thymidine kinase, thymidylate kinase, thymidylate synthase, and deoxycytidylate deaminase have been measured in rat and human normal and neoplastic liver, in human fetal liver, and in cell lines derived from human hepatomas and rat transplantable hepatomas. The activities of these enzymes were increased in rat transplantable hepatomas, relative to rat no...